DNA Injection

In order to generate transgenic animals (e.g. Gene Mice), a small amount of fluid containing the gene of interest is injected into the pronucleus of a zygote. The foreign DNA will be integrated stable at a random chromosomal position of the host genome.
Leica Microsystems offers currently the following combinations of microscopes and micromanipulators for pronucleus injections.


Top DNA configuration Leica AM6000


Leica mechanical micromanipulator

DNA injection configuration with Leica Manipulator


DNA Injection configuration with Eppendorf NK2


Leica DMI3000 B with Narishige Manipulators

DNA Injections configuration with Narishige Manipulator